What Anime moment did Du find saddest?

(For me, I have several ones: When Ace is killed Von Akainu in One Piece and Luffy cries his little herz out, then loses his mind from despair; when Spike had finally met up with Julia and then she is killed Von the Syndicate in Cowboy Bebop, as well as Spike then dying a few episodes later; when Otonashi and Kanade are separated, right at the end of Angel – Jäger der Finsternis Beats... Kanade disappears, but Otonashi stays; when gin Ichimaru was killed Von Sosuke Aizen in Bleach, then Rangiku finds his dying body and is cut up about it; when Coyote Starrk, an Espada in Bleach, is killed Von his opponent, even though didn't really want to fight... He had to follow orders; lastly, near the end of K-On when Azunyan realises that Yui, Mio, Ricchan and Mugi will be at college and she'll be alone.)