Can Du lose your viginity while using a vibrator?

No.
That only happens when your having intercourse with someone.

First I must know what Virginity is.

To lose your virginity Du have to have sex. So to answer your question: no.

Depends on who Du talk to.
I strongly believe to be a non-virgin is have proper sex, as in dick in vagina.
But I've spoken to one person who believe it's just pure penetration of any kind. But Von definition it is pure heterosexual sex.

I don't think so.